Recombinant人DNA PKcs protein (ab114159)

概述

描述

  • 性质
    Recombinant
  • 来源
    Wheat germ
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 序列
      KMLKKGGSWIQEINVAEKNWYPRQKICYAKRKLAGANPAVITCDELLLGH EKAPAFRDYVAVARGSKDHNIRAQEPESGLSEETQVKCLMDQATDPNILG RTWEGWEPWM
    • 分子量
      38 kDa including tags
    • 氨基酸
      4019 to 4128
    • 标签
      GST tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab114159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    ELISA

    Western blot

    SDS-PAGE

  • 形式
    Liquid
  • 补充说明
    Protein concentration is above or equal to 0.05 mg/ml.
    ab114159 is best used within three months from the date of receipt.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

常规信息

  • 别名
    • DNA dependent protein kinase catalytic subunit
    • DNA PK catalytic subunit
    • DNA-dependent protein kinase catalytic subunit
    • DNA-PK catalytic subunit
    • DNA-PKcs
    • DNAPK
    • DNAPK catalytic subunit
    • DNPK 1
    • DNPK1
    • Hyper radiosensitivity of murine scid mutation, complementing 1
    • Hyperradiosensitivity complementing 1, mouse, homolog of 1
    • HYRC
    • HYRC 1
    • HYRC1
    • IMD26
    • p350
    • p460
    • PKRDC
    • PRKDC
    • PRKDC_HUMAN
    • Protein Kinase DNA Activated Catalytic Polypeptide
    • XRCC 7
    • XRCC7
    see all
  • 功能
    Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.
  • 序列相似性
    Belongs to the PI3/PI4-kinase family.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 2 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
    Contains 3 TPR repeats.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 and Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site (inducible with ionizing radiation, IR). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair.
    S-nitrosylated by GAPDH.
  • 细胞定位
    Nucleus.
  • Information by UniProt

图片

  • 12.5% SDS-PAGE analysis of ab114159, stained with Coomassie Blue.

文献

ab114159 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab114159.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册