Recombinant人DLL4 protein (Fc Chimera Active) (ab108557)

概述

描述

  • 性质Recombinant
  • 来源HEK 293 cells
  • 氨基酸序列
    • AccessionQ9NR61
    • 种属Human
    • 分子量80 kDa
    • 氨基酸1 to 529
    • 额外的序列信息Fused to the Fc region of Human IgG1 at the C-terminus.

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技术指标

Our Abpromise guarantee covers the use of ab108557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    Inhibits adipogenesis of 3T3L-1 cells and mesenchymal stem cells (MSCs). Induces Hes-1 in 3T3L-1 cells.

  • 应用

    Functional Studies

    SDS-PAGE

  • 内毒素水平< 0.100 Eu/µg
  • 纯度> 90 % SDS-PAGE.
    ab108557 is 0.2 µm filtered.
  • 形式Lyophilised
  • 补充说明

    Working aliquots are stable for up to 3 months when stored at -20°C. ab108557 interacts with Human Notch1 (as confirmed by Flow Cytometry).

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Constituents: PBS, 0.5% Trehalose

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶10µg size: Reconstitute with 100µl sterile water

常规信息

  • 别名
    • Delta 4
    • delta 4 precursor
    • Delta ligand 4
    • delta ligand 4 precursor
    • Delta like 4
    • Delta like 4 homolog
    • Delta like 4 protein
    • Delta like protein 4
    • Delta-like 4 (Drosophila)
    • Delta-like protein 4
    • Delta4
    • DLL 4
    • Dll4
    • DLL4_HUMAN
    • Drosophila Delta homolog 4
    • hdelta2
    • Homeobox protein DLL-4
    • MGC126344
    • Notch ligand delta 2
    • Notch ligand DLL4
    • Notch ligand DLL4 precursor
    • XDLL-4
    see all
  • 功能Plays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4.
  • 组织特异性Expressed in vascular endothelium.
  • 序列相似性Contains 1 DSL domain.
    Contains 8 EGF-like domains.
  • 结构域The Delta-Serrate-Lag2 (DSL) domain is required for binding to the Notch receptor.
  • 翻译后修饰Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • 细胞定位Membrane.
  • Information by UniProt

Recombinant human DLL4 protein (Fc Chimera Active) 图像

  • Adipogenesis inhibition of 3T3L-1 cells.
    3T3L-1 cells (mouse pre-adipocyte cells) were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For differentiation of 3T3L-1 cells, 3T3L-1 cells were cultured in adipogenic medium which was growth medium supplemented with 1 µM Dexamethasone, 0.5 mM IBMX, 10 µg/ml lnsulin (day 0). Medium was changed every 2 days. Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol. For negative controls, mouse TNF-a (20 ng/ml) was added. Recombinant Human DLL4-Fc (ab108557) (5 µg/ml) dissolved in DPBS was added to the differentiation medium. These plates were then used to differentiate 3T3L-1 cells.
  • Adipogenesis inhibition of MSCs.
    MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilinstreptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1 µM Dexamethasone, 0.5mM IBMX, 10 µg/m lnsulin, 100 µM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-a (20 ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were incubated with a solution of ab108557 (5 µg/ml) or mCD137-Fc (5 µg/ml) in PBS for 2 hours at 37°C. Plates were then used to differentiate MSCs.
  • Interaction of Human Notch1 with Human DLL4.
    HEK293 cells transfected with a Human Notch1 or a Human GITR ligand expressing vector were incubated with 25 µg/ml of Human GITR-Fc or ab108557. Cells were stained with anti-Human IgG (Fc specific) FITC conjugate for DLL4-Fc binding.
  • Adipogenesis inhibition of 3T3L-1 cells.
  • Induction of Hes-1 with the treatment of recombinant Human DLL4-Fc (ab108557).
    A Mouse preadpipocyte cell line, 3T3L-1, was stimulated with 5 µg/ml of Human DLL4-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-Mouse Hes1 or GAPDH.

    M: Marker
    1: hDLL4-Fc, 0 min
    2: hDLL4-Fc, 10 min
    3: hDLL4-Fc, 30 min
    4: hDLL4-Fc, 1 hr
    5: hDLL4-Fc, 2 hr
    6: hDLL4-Fc, 4 hr
    7: hDLL4-Fc, 8 hr
    8: hDLL4-Fc, 24 hr
  • 50 µg of cell lysates derived from hDLL4-Fc (ab108557) or non-treated 3T3L-1 cells, which had been either differentiated or undifferentiated, were subjected to Western blot by using a Mouse adiponectin antibody.

Recombinant human DLL4 protein (Fc Chimera Active) (ab108557)参考文献

This product has been referenced in:
  • Faronato M  et al. DMXL2 drives epithelial to mesenchymal transition in hormonal therapy resistant breast cancer through Notch hyper-activation. Oncotarget 6:22467-79 (2015). Read more (PubMed: 26093085) »
  • Hale AT  et al. Endothelial Kruppel-like factor 4 regulates angiogenesis and the Notch signaling pathway. J Biol Chem 289:12016-28 (2014). Read more (PubMed: 24599951) »

See all 2 Publications for this product

Product Wall

Thank you for your reply.

I am confident that the reference ab90285 is a suitable negative control for ab108557.
Furthermore, I have just been informed about the price of the new Fc fragments which is $424.00 for 100µg, therefore I w...

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I would like to inform you that the originator of the DLL4 fused to the Fc portion of Human IgG1 (ab108557) is able to supply the Fc portion only.

Could you please let me know if you would still be interested in such product? If so we will add...

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Thank you very much for contacting us.

I just wanted to let you know that I am still working on your inquiry.

I have contacted the originator of the DLL4 protein ab108557 who informed me thatthe Human IgG Fc fragment we have in our ca...

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Thank you very much for contacting us with your question.

I've looked over both of these products, and they are both active DLL4 proteins. I'm not sure why ab108557 does not have "active" in its name, but I will recommend that this ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"