Recombinant人DLL1 protein (ab108576)

概述

描述

  • 性质
    Recombinant
  • 来源
    HEK 293 cells
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 分子量
      70 kDa including tags
    • 氨基酸
      1 to 545
    • 标签
      DDDDK tag C-Terminus

技术指标

Our Abpromise guarantee covers the use of ab108576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    Inhibits adipogenesis of mesenchymal stem cells.
  • 应用

    Functional Studies

    SDS-PAGE

  • 内毒素水平
    < 0.100 Eu/µg
  • 纯度
    > 90 % SDS-PAGE.
    ab108576 is 0.2µm filtered
  • 形式
    Liquid
  • 补充说明
    Working aliquots are stable for up to 3 months when stored at -20°C. Inhibits adipogenesis of mesenchymal stem cells.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS

常规信息

  • 别名
    • Delta
    • Delta (Drosophila) like 1
    • Delta like 1
    • Delta like 1 (Drosophila)
    • Delta like 1 protein
    • Delta like protein 1
    • Delta like protein 1 precursor
    • Delta, Drosophila, homolog of 1
    • Delta-like protein 1
    • Delta1
    • DL1
    • DLL 1
    • DLL1
    • DLL1_HUMAN
    • Drosophila Delta homolog 1
    • H-Delta-1
    • OTTHUMP00000017690
    see all
  • 功能
    Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
  • 组织特异性
    Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
  • 序列相似性
    Contains 1 DSL domain.
    Contains 8 EGF-like domains.
  • 翻译后修饰
    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • 细胞定位
    Membrane.
  • Information by UniProt

图片

  • Adipogenesis inhibition of MSCs.
    MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1µM Dexamethasone, 0.5mM IBMX, 10µg/m lnsulin, 100µM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-alpha (20ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were first incubated with a solution of anti-FLAG® antibody (10µg/ml) in PBS for 30 mins at 37°C. Coated plates were blocked with growth medium for at least 30mins, incubated with solution of ab108576 (5µg/ml) or mCD137L-FLAG® (5µg/ml) in growth medium for 2 hours at 37°C. Plates were then used to differentiate MSCs.

文献

ab108576 has not yet been referenced specifically in any publications.

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