序列LLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQ KNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLI SGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAI L
功能Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.
疾病相关Pleuropulmonary blastoma Goiter multinodular 1, with or without Sertoli-Leydig cell tumors Rhabdomyosarcoma, embryonal, 2 DICER1 mutations have been found in uterine cervix embryonal rhabdomyosarcoma, primitive neuroectodermal tumor, Wilms tumor, pulmonary sequestration and juvenile intestinal polyp (PubMed:21882293). Somatic missense mutations affecting the RNase IIIb domain of DICER1 are common in non-epithelial ovarian tumors. These mutations do not abolish DICER1 function but alter it in specific cell types, a novel mechanism through which perturbation of microRNA processing may be oncogenic (PubMed:22187960).
SDS-PAGE - Dicer protein (Tagged-His Tag) (ab92268)
The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.
Recombinant Human Dicer protein (ab92268)参考文献
has not yet been referenced specifically in any publications.