Recombinant人Cytokeratin 8 protein (ab73641)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • 种属
      Human

技术指标

Our Abpromise guarantee covers the use of ab73641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度
    > 95 % SDS-PAGE.
    ab73641 is purified by proprietary chromatographic techniques. Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
  • 形式
    Lyophilised
  • 补充说明
    Reconstitution to filaments: Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCI, 2 mM dithiothreitol, 10 mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 9.5M Urea, 30mM Tris HCl, 10mM methylammonium chloride, 2mM EDTA, 2mM DTT, pH 8

  • 复溶
    Reconstitute in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

常规信息

  • 别名
    • CARD2
    • CK 8
    • CK-8
    • CK8
    • CYK8
    • CYKER
    • Cytokeratin endo A
    • Cytokeratin-8
    • DreK8
    • EndoA
    • K2C8
    • K2C8_HUMAN
    • K8
    • Keratin 8
    • Keratin type II cytoskeletal 8
    • Keratin, type II cytoskeletal 8
    • Keratin-8
    • KO
    • Krt 2.8
    • KRT8
    • MGC118110
    • MGC174782
    • MGC53564
    • MGC85764
    • sb:cb186
    • Type-II keratin Kb8
    see all
  • 功能
    Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • 组织特异性
    Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
  • 疾病相关
    Cirrhosis
  • 序列相似性
    Belongs to the intermediate filament family.
  • 翻译后修饰
    Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
    O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • 细胞定位
    Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
  • Information by UniProt

文献

ab73641 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab73641.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册