Recombinant人Cytokeratin 20 protein (ab73640)



  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 分子量49 kDa
    • 额外的序列信息Cytokeratin 20 Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain having a molecular mass of 48,553 Dalton.


Our Abpromise guarantee covers the use of ab73640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用


    Western blot

  • 纯度> 95 % SDS-PAGE.
    ab73640 is purified by proprietary chromatographic techniques. Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
  • 形式Lyophilised
  • 补充说明Reconstitution to filaments: Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCI, 2 mM dithiothreitol, 10 mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
  • Concentration information loading...


  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 9.5M Urea, 30mM Tris HCl, 10mM methylammonium chloride, 2mM EDTA, 2mM DTT, pH 8

  • 复溶Reconstitute in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.


  • 别名
    • CD20
    • CK 20
    • CK-20
    • CK20
    • Cytokeratin-20
    • Cytokeratin20
    • K1C20_HUMAN
    • K20
    • KA20
    • Keratin
    • Keratin 20
    • keratin 20, type I
    • keratin 21, rat, homolog of
    • Keratin type I cytoskeletal 20
    • Keratin-20
    • Keratin20
    • KRT 20
    • KRT 21
    • KRT20
    • KRT21
    • MGC35423
    • OTTHUMP00000164518
    • Protein IT
    • type I cytoskeletal 20
    see all
  • 功能Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
  • 组织特异性Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
  • 序列相似性Belongs to the intermediate filament family.
  • 发展阶段First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.
  • 翻译后修饰Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
    Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
  • 细胞定位Cytoplasm.
  • Information by UniProt

Recombinant Human Cytokeratin 20 protein (ab73640)参考文献

ab73640 has not yet been referenced specifically in any publications.

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