Recombinant人Cytokeratin 19 protein (ab73639)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab73639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Western blot

  • 纯度> 95 % SDS-PAGE.
    ab73639 is purified by proprietary chromatographic techniques. Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
  • 形式Lyophilised
  • 补充说明Reconstitution to filaments: Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCI, 2 mM dithiothreitol, 10 mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 9.5M Urea, 30mM Tris HCl, 10mM methylammonium chloride, 2mM EDTA, pH 8

  • 复溶Reconstitute in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

常规信息

  • 别名
    • 40 kDa keratin intermediate filament
    • CK 19
    • CK-19
    • CK19
    • Cytokeratin 19
    • Cytokeratin-19
    • K19
    • K1C19_HUMAN
    • K1CS
    • Keratin 19
    • Keratin type I 40 kD
    • Keratin type I 40kD
    • Keratin type I cytoskeletal 19
    • Keratin, type I cytoskeletal 19
    • Keratin, type I, 40 kd
    • Keratin-19
    • KRT19
    • MGC15366
    see all
  • 功能Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • 组织特异性Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
  • 序列相似性Belongs to the intermediate filament family.
  • 发展阶段Present in hair follicles at all stages of development.
  • 结构域This keratin differs from all other IF proteins in lacking the C-terminal tail domain.
  • Information by UniProt

Recombinant Human Cytokeratin 19 protein 图像

  • Anti-Cytokeratin 19 antibody (ab85351) at 1 µg/ml + Recombinant Human Cytokeratin 19 protein (ab73639) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

Recombinant Human Cytokeratin 19 protein (ab73639)参考文献

ab73639 has not yet been referenced specifically in any publications.

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