Recombinant人Cytochrome P450 3A4 protein (ab114327)

概述

描述

  • 性质Recombinant
  • 来源Wheat germ
  • 氨基酸序列
    • AccessionP08684
    • 种属Human
    • 序列MALIPDLAMETWLLLAVSLVLLYLYGTHSHGLFKKLGIPGPTPLPFLGNI LSYHKGFCMFDMECHKKYGKVWGFYDGQQPVLAITDPDMIKTVLVKECYS VFTNRRPFGPVGFMKSAISIAEDEEWKRLRSLLSPTFTSGKLKEMVPIIA QYGDVLVRNLRREAETGKPVTLKDVFGAYSMDVITSTSFGVNIDSLNNPQ DPFVENTKKLLRFDFLDPFFLSITVFPFLIPILEVLNICVFPREVTNFLR KSVKRMKESRLEDTQKHRVDFLQLMIDSQNSKETESHKALSDLELVAQSI IFIFAGYETTSSVLSFIMYELATHPDVQQKLQEEIDAVLPNKAPPTYDTV LQMEYLDMVVNETLRLFPIAMRLERVCKKDVEINGMFIPKGVVVMIPSYA LHRDPKYWTEPEKFLPERFSKKNKDNIDPYIYTPFGSGPRNCIGMRFALM NMKLALIRVLQNFSFKPCKETQIPLKLSLGGLLQPEKPVVLKVESRDGTV SGA
    • 分子量81 kDa
    • 氨基酸1 to 503

技术指标

Our Abpromise guarantee covers the use of ab114327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    SDS-PAGE

    ELISA

  • 形式Liquid
  • 补充说明Protein concentration is above or equal to 0.05 mg/ml. Best use within three months from the date of receipt of this protein.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

常规信息

  • 别名
    • 1,8-cineole 2-exo-monooxygenase
    • Albendazole monooxygenase
    • Albendazole sulfoxidase
    • CP33
    • CP34
    • CP3A4_HUMAN
    • CYP3
    • CYP3A
    • CYP3A3
    • CYP3A4
    • CYPIIIA3
    • CYPIIIA4
    • Cytochrome P450 3A3
    • Cytochrome P450 3A4
    • Cytochrome P450 family 3 subfamily A polypeptide 4
    • Cytochrome P450 HLp
    • Cytochrome P450 NF-25
    • Cytochrome P450 subfamily IIIA polypeptide 4
    • cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3
    • cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4
    • Cytochrome P450-PCN1
    • Glucocorticoid inducible P450
    • HLP
    • MGC126680
    • NF 25
    • NF25
    • Nifedipine oxidase
    • P450 III steroid inducible
    • P450 PCN1
    • P450, family III
    • P450C3
    • P450PCN1
    • Quinine 3 monooxygenase
    • Quinine 3-monooxygenase
    • Taurochenodeoxycholate 6 alpha hydroxylase
    • Taurochenodeoxycholate 6-alpha-hydroxylase
    see all
  • 功能Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide.
  • 组织特异性Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056). According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level).
  • 序列相似性Belongs to the cytochrome P450 family.
  • 翻译后修饰Polyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro).
  • 细胞定位Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt

Recombinant Human Cytochrome P450 3A4 protein 图像

  • SDS-PAGE analysis of ab114327 on a 12.5% gel stained with Coomassie Blue.
  • Anti-Cytochrome P450 3A4 antibody (ab94334) at 1 µg/ml + Recombinant Human Cytochrome P450 3A4 protein (ab114327) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 seconds

Recombinant Human Cytochrome P450 3A4 protein (ab114327)参考文献

ab114327 has not yet been referenced specifically in any publications.

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