The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
生物活性Chemotaxis effect of soluble recombinant Human CX3CL1 in THP-1 cells.
内毒素水平< 0.100 Eu/µg
% SDS-PAGE. ab108577 is 0.2µm filtered
补充说明Working aliquots are stable for up to 3 months when stored at -20°C.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
C-X3-C motif chemokine 1
Chemokine (C-X3-C motif) ligand 1
Chemokine C X3 C motif ligand 1
Chemokine CX3C Motif Ligand 1
CX3C membrane anchored chemokine
CX3C membrane-anchored chemokine
Small inducible cytokine D1
Small inducible cytokine subfamily D (Cys X3 Cys) member 1
small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin)
Small inducible cytokine subfamily D member 1
Small-inducible cytokine D1
功能The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.
Chemotaxis effect of human CX3CL1-DDDDK (ab108577) in THP-1 Cells.
Cell migration of THP-1 monocytic leukemia cells was evaluated in disposable 24 well transwell polystyrene membrane with 8µm size pores. 100ng, 500ng, 1,000ng, 2,500ng and 5,000ng of recombinant CX3CL1 were diluted in RPMI 1640 supplemented with 10mg/ml BSA, respectively. CX3CL1 samples were added to the lower chamber. THP-1 cells (0.5 X 106 cells per well), in the same media with ab108577, were added to the upper chamber. After 2 hour incubation at 37°C in 5% CO2 humidified atmosphere, migration cells in the lower chamber were counted. Migrated cells in two separate fields per well from duplicate wells were enumerated on a hemacytometer by means light microscopy.
Recombinant human CX3CL1 protein (ab108577)参考文献
has not yet been referenced specifically in any publications.