Recombinant人CSK protein (ab42617)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab42617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific Activity : 100 U/mg. One unit defined as the amount of enzyme that will transfer 1nmol phosphate to Tyr substrate per minute at pH 7.4 and 30deg.C. Assay buffer : 50mM HEPES pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3um Na-orthovanadate, 0.1M ATP, 30ug/ml Poly (Glu:Tyr) 4:1 substrate and 1ug/ml recombinant CSK.
  • 应用

    Inhibition Assay

  • 纯度> 90 % SDS-PAGE.

  • 形式Liquid
  • 补充说明Source : Baculovirus infected Sf9 cells
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 30% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, 10mM reduced Glutathione, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • C SRC
    • C SRC kinase
    • C src Tyrosine Kinase
    • C-SRC kinase
    • c-src tyrosine kinase
    • CSK
    • Csk A
    • CSK_HUMAN
    • CYTOPLASMIC TYROSINE KINASE
    • EC 2.7.10.2
    • MGC112926
    • MGC117393
    • MGC154049
    • P60 Src
    • Protein tyrosine kinase CYL
    • Protein-tyrosine kinase CYL
    • Proto oncogene tyrosine protein kinase
    • Tyrosine protein kinase CSK
    • Tyrosine-protein kinase CSK
    • zgc:154049
    see all
  • 功能Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
  • 组织特异性Expressed in lung and macrophages.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻译后修饰Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.
  • 细胞定位Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
  • Information by UniProt

Recombinant human CSK protein (ab42617)参考文献

ab42617 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab42617.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"