Recombinant人CRHBP protein (ab101052)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP24387
    • 种属Human
    • 序列MKHHHHHHASYLELREAADYDPFLLFSANLKRELAGEQPYRRALRCLDML SLQGQFTFTADRPQLHCAAFFISEPEEFITIHYDQVSIDCQGGDFLKVFD GWILKGEKFPSSQDHPLPSAERYIDFCESGLSRRSIRSSQNVAMIFFRVH EPGNGFTLTIKTDPNLFPCNVISQTPNGKFTLVVPHQHRNCSFSIIYPVV IKISDLTLGHVNGLQLKKSSAGCEGIGDFVELLGGTGLDPSKMTPLADLC YPFHGPAQMKVGCDNTVVRMVSSGKHVNRVTFEYRQLEPYELENPNGNSI GEFCLSGL
    • 分子量35 kDa including tags
    • 氨基酸25 to 322
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab101052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    ELISA

    SDS-PAGE

  • 形式Lyophilised
  • 补充说明Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20mM Sodium chloride, 20mM Tris, pH 7.5

  • 复溶Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

常规信息

  • 别名
    • Corticotropin releasing factor binding protein
    • Corticotropin releasing hormone binding protein
    • CRF binding protein
    • CRF BP
    • CRFBP
    • CRH BP
    see all
  • 相关性CRHBP is a potent stimulator of synthesis and secretion of preopiomelanocortin derived peptides. Although CRH concentrations in the human peripheral circulation are normally low, they increase throughout pregnancy and fall rapidly after parturition. Maternal plasma CRH probably originates from the placenta. Human plasma contains a CRH binding protein which inactivates CRH and which may prevent inappropriate pituitary adrenal stimulation in pregnancy.
  • 细胞定位Extracellular space

Recombinant Human CRHBP protein 图像

  • 14% SDS-PAGE analysis of ab101052
    Lane 1: Molecular weight markers 97, 66, 45, 31, 21, 14 kDa
    Lane 2: ab101052 (reduced and boiled, 5µg/lane)
    lane 3: ab101052 (non-reduced and non-boiled, 5µg/lane)

Recombinant Human CRHBP protein (ab101052)参考文献

ab101052 has not yet been referenced specifically in any publications.

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