Recombinant人Creatine Kinase MM protein (ab73652)

概述

描述

  • 性质
    Recombinant
  • 来源
    Pichia pastoris
  • 氨基酸序列
    • 种属
      Human
    • 额外的序列信息
      CKMT1 Human Recombinant without C-terminal Lysine on both chains produced in Pichia Pastoris is a glycosylated 47kDa polypeptide chain having an identical amino acid sequence to the native enzyme.

技术指标

Our Abpromise guarantee covers the use of ab73652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 µmole creatine phosphate was 500IU/mg at 37 degrees celsius.
  • 应用

    ELISA

    SDS-PAGE

  • 纯度
    > 95 % SDS-PAGE.
    ab73652 is purified by proprietary chromatographic techniques under non-denaturing conditions. Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: 0.1% Sodium Azide
    Constituents: 50% Glycerol, 0.1M Tris HCl, 0.075M Sodium chloride, 10mM Beta mercaptoethanol, pH 7.2

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • CKM
    • CKMM
    • Creatine kinase M
    • Creatine kinase M chain
    • Creatine kinase M type
    • Creatine kinase M-type
    • Creatine kinase muscle
    • Creatine kinase, muscle type
    • KCRM_HUMAN
    • M-CK
    • MCK
    • Muscle creatine kinase
    see all
  • 功能
    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • 序列相似性
    Belongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt

文献

ab73652 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your inquiry. The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 µmole creatine phosphate was 537IU/mg at 37 degrees celsius. I hope this information helps. Please let me ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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