Recombinant人COX2 / Cyclooxygenase 2 protein (ab58868)

概述

描述

  • 性质
    Recombinant
  • 来源
    Baculovirus infected Sf21 cells
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 标签
      His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab58868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    Specific Activity: minimum 8,000 units per mg protein
  • 应用

    Functional Studies

    SDS-PAGE

    Western blot

    Other

  • 形式
    Liquid
  • 补充说明
    This protein is relatively unstable at room temperature; Keep the enzyme on ice (0 °C to 4 °C) at all times.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 1.261% Tris HCl, 0.1% Tween, 0.00675% Diethyldithiocarbamate, 10% Glycerol

    If this preservative (DDC) is undesirable, it can be removed by standard desalting procedures, but the enzyme is unstable in the absence of the preservative and so should be used within one hour.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • COX 2
    • COX-2
    • COX2
    • Cyclooxygenase
    • Cyclooxygenase 2
    • Cyclooxygenase 2b
    • Cyclooxygenase-2
    • Cyclooxygenase2
    • EC 1.14.99.1
    • fj02a10
    • Glucocorticoid-regulated inflammatory cyclooxygenase
    • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase
    • GRIPGHS
    • hCox 2
    • Macrophage activation-associated marker protein P71/73
    • OTTHUMP00000033524
    • PES-2
    • PGG/HS
    • PGH synthase 2
    • PGH2_HUMAN
    • PGHS 2
    • PGHS-2
    • PGHS2
    • PHS 2
    • PHS II
    • PHS2
    • Prostaglandin endoperoxide synthase 2
    • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
    • Prostaglandin G/H synthase
    • Prostaglandin G/H synthase 2
    • Prostaglandin G/H synthase 2 precursor
    • Prostaglandin G/H synthase and cyclooxygenase
    • Prostaglandin H2 synthase 2
    • Prostaglandin-endoperoxide synthase 2
    • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
    • PTGS2
    • ptgs2a
    • TIS10
    • TIS10 protein
    • unp1239
    • wu:fj02a10
    see all
  • 功能
    Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
  • 通路
    Lipid metabolism; prostaglandin biosynthesis.
  • 序列相似性
    Belongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • 翻译后修饰
    S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
  • 细胞定位
    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt

图片

  • Standard curve for COX2 / Cyclooxygenase 2 (Analyte: ab58868); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab90345 at 1µg/ml and Detector Antibody ab15191 at 0.5µg/ml.

  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

文献

ab58868 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
This recombinant protein ran one clean band detected by ab15191 Cox2 antibody. Worked perfectly for our needs
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提交于 May 05 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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