The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
生物活性Induces Hes-1 in 3T3L-1 cells.
内毒素水平< 0.100 Eu/µg
% SDS-PAGE. ab109345 is an 0.2 µm filtered solution.
Induces Hes-1 in 3T3L-1 cells.
Concentration information loading...
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Contactin 6 precursor
Neural adhesion molecule
Neural recognition molecule NB 3
Neural recognition molecule NB-3
功能Contactins mediate cell surface interactions during nervous system development. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Involved in motor coordination.
组织特异性Expressed in nervous system. Highly expressed in cerebellum. Expressed at intermediate level in thalamus, subthalamic nucleus. Weakly expressed in corpus callosum, caudate nucleus and spinal cord.
序列相似性Belongs to the immunoglobulin superfamily. Contactin family. Contains 4 fibronectin type-III domains. Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Functional Studies - Contactin 6 protein (ab109345)
Induction of Hes-1 with the treatment of hContactin-6-Fc.
A Mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 µg/ml of ab109345 as in indicated time points and each cell lysate was prepared and subjected to Western blot by using anti-Mouse Hes-1 or GAPDH (loading control).
Lane M: Marker
Lane 1: ab109345, 0 minutes
Lane 2: ab109345, 10 minutes
Lane 3: ab109345, 30 minutes
Lane 4: ab109345, 1 h
Lane 5: ab109345, 2 h
Lane 6: ab109345, 4 h
Lane 7: ab109345, 8 h
Lane 8: ab109345, 24 h
Recombinant Human Contactin 6 protein (ab109345)参考文献
has not yet been referenced specifically in any publications.