重组人Contactin 6蛋白(ab109345)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人Contactin 6蛋白
参阅全部 Contactin 6 蛋白酶 -
生物活性
Induces Hes-1 in 3T3L-1 cells. -
纯度
> 90 % SDS-PAGE.
ab109345 is an 0.2 µm filtered solution. -
内毒素水平
< 0.100 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
150 kDa including tags -
氨基酸
1 to 999
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技术指标
Our Abpromise guarantee covers the use of ab109345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
补充说明
Induces Hes-1 in 3T3L-1 cells. -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituent: PBS
常规信息
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别名
- CNTN 6
- CNTN6
- CNTN6_HUMAN
see all -
功能
Contactins mediate cell surface interactions during nervous system development. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Involved in motor coordination. -
组织特异性
Expressed in nervous system. Highly expressed in cerebellum. Expressed at intermediate level in thalamus, subthalamic nucleus. Weakly expressed in corpus callosum, caudate nucleus and spinal cord. -
序列相似性
Belongs to the immunoglobulin superfamily. Contactin family.
Contains 4 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
细胞定位
Cell membrane. - Information by UniProt
图片
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Induction of Hes-1 with the treatment of hContactin-6-Fc.
A Mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 μg/ml of ab109345 as in indicated time points and each cell lysate was prepared and subjected to Western blot by using anti-Mouse Hes-1 or GAPDH (loading control).Lane M: Marker
Lane 1: ab109345, 0 minutes
Lane 2: ab109345, 10 minutes
Lane 3: ab109345, 30 minutes
Lane 4: ab109345, 1 h
Lane 5: ab109345, 2 h
Lane 6: ab109345, 4 h
Lane 7: ab109345, 8 h
Lane 8: ab109345, 24 h
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab109345 尚未被引用在任何文献中。