重组人Cofilin蛋白(ab85154)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% Densitometry
- Suitable for: SDS-PAGE, WB
描述
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产品名称
重组人Cofilin蛋白
参阅全部 Cofilin-1 蛋白酶 -
纯度
> 95 % Densitometry. -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human
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相关产品
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Recombinant Protein
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab85154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Western blot
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形式
Liquid -
补充说明
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.0308% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride
常规信息
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别名
- 18 kDa phosphoprotein
- CFL
- CFL 1
see all -
功能
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. -
组织特异性
Widely distributed in various tissues. -
序列相似性
Belongs to the actin-binding proteins ADF family.
Contains 1 ADF-H domain. -
翻译后修饰
Phosphorylated on Ser-3 in resting cells. -
细胞定位
Nucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab85154 被引用在 1 文献中.
- Lu H et al. Curcolonol suppresses the motility of breast cancer cells by inhibiting LIM kinase 1 to downregulate cofilin 1 phosphorylation. Int J Oncol 53:2695-2704 (2018). PubMed: 30320377