Recombinant人Clusterin protein (ab69754)

概述

描述

  • 性质Recombinant
  • 来源HEK 293 cells
  • 氨基酸序列
    • 种属Human
    • 序列DQTVSDNELQ EMSNQGSKYV NKEIQNAVNG VKQIKTLIEK TNEERKTLLS NLEEAKKKKE DALNETRESE TKLKELPGVC NETMMALWEE CKPCLKQTCM KFYARVCRSGS GLVGRQLEE FLNQSSPFYF WMNGDRIDSL LENDRQQTHM LDVMQDHFSRA SSIIDELFQ DRFFTREPQD TYHYLPFSLP HRRPHFFFPK SRIVRSLMPF SPYEPLNFHA MFQPFLEMIH EAQQAMDIHF HSPAFQHPPT EFIREGDDDR TVCREIRHNS TGCLRMKDQC DKCREILSVD CSTNNPSQAKLRRELDESLQ VAERLTRKYN ELLKSYQWKM LNTSSLLEQL NEQFNWVSRL ANLTQGEDQYYLRVTTVASH TSDSDVPSGV TEVVVKLFDS DPITVTVPVE VSRKNPKFME TVAEKALQEY RKKHREEGSL GSGWSHPQFE KTGHHHHHHH HGGQ
    • 分子量51 kDa
    • 氨基酸1 to 427
    • 标签DDDDK tag C-Terminus

技术指标

Our Abpromise guarantee covers the use of ab69754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    Purified by Ni-NTA chromatography. Purity is greater than 95% (SDS PAGE analyzed). Filtered (0.4 micron).
  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS, pH 7.5

  • 复溶Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles.

常规信息

  • 别名
    • 40
    • AAG 4
    • AAG4
    • Aging associated protein 4
    • Aging-associated gene 4 protein
    • AI893575
    • APO J
    • Apo-J
    • APOJ
    • ApoJalpha
    • ApoJbeta
    • Apolipoprotein J
    • ApolipoproteinJ
    • CLI
    • CLU
    • CLU1
    • CLU2
    • CLUS_HUMAN
    • Clusterin
    • Clusterin alpha chain
    • Clusterin beta chain
    • Complement associated protein SP 40
    • Complement associated protein SP 40 40
    • Complement associated protein SP40
    • Complement cytolysis inhibitor
    • Complement cytolysis inhibitor a chain
    • Complement cytolysis inhibitor b chain
    • Complement lysis inhibitor
    • Complement-associated protein SP-40
    • D14Ucla3
    • Dimeric acid glycoprotein
    • Glycoprotein 80
    • Glycoprotein III
    • GP80
    • Ku70-binding protein 1
    • KUB 1
    • KUB1
    • MGC24903
    • NA1/NA2
    • RATTRPM2B
    • SGP 2
    • SGP2
    • SP 40
    • SP40
    • Sugp-2
    • Sulfated glycoprotein 2
    • Testosterone repressed prostate message 2
    • Testosterone-repressed prostate message 2
    • TRPM 2
    • TRPM-2
    • TRPM2
    • TRPM2B
    • Trpmb
    see all
  • 功能Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
  • 组织特异性Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.
  • 序列相似性Belongs to the clusterin family.
  • 翻译后修饰Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
    Polyubiquitinated, leading to proteasomal degradation.
    Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.
  • 细胞定位Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis.
  • Information by UniProt

Recombinant Human Clusterin protein (ab69754)参考文献

ab69754 has not yet been referenced specifically in any publications.

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