Recombinant人Clusterin protein (ab109150)

概述

描述

  • 性质
    Recombinant
  • 来源
    HEK 293 cells
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 分子量
      80 kDa including tags
    • 氨基酸
      1 to 449
    • 标签
      DDDDK tag C-Terminus

技术指标

Our Abpromise guarantee covers the use of ab109150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 内毒素水平
    < 0.100 Eu/µg
  • 纯度
    > 90 % SDS-PAGE.
    ab109150 is 0.2µm filtered
  • 形式
    Liquid
  • 补充说明
    Working aliquots are stable for up to 3 months when stored at -20°C.


    The secreted form of Apolipoprotein J is a heterodimeric disulfide-linked glycoprotein of 76–80 kDa.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS, pH 7.2

常规信息

  • 别名
    • 40
    • AAG 4
    • AAG4
    • Aging associated protein 4
    • Aging-associated gene 4 protein
    • AI893575
    • APO J
    • Apo-J
    • APOJ
    • ApoJalpha
    • ApoJbeta
    • Apolipoprotein J
    • ApolipoproteinJ
    • CLI
    • CLU
    • CLU1
    • CLU2
    • CLUS_HUMAN
    • Clusterin
    • Clusterin alpha chain
    • Clusterin beta chain
    • Complement associated protein SP 40
    • Complement associated protein SP 40 40
    • Complement associated protein SP40
    • Complement cytolysis inhibitor
    • Complement cytolysis inhibitor a chain
    • Complement cytolysis inhibitor b chain
    • Complement lysis inhibitor
    • Complement-associated protein SP-40
    • D14Ucla3
    • Dimeric acid glycoprotein
    • Glycoprotein 80
    • Glycoprotein III
    • GP80
    • Ku70-binding protein 1
    • KUB 1
    • KUB1
    • MGC24903
    • NA1/NA2
    • RATTRPM2B
    • SGP 2
    • SGP2
    • SP 40
    • SP40
    • Sugp-2
    • Sulfated glycoprotein 2
    • Testosterone repressed prostate message 2
    • Testosterone-repressed prostate message 2
    • TRPM 2
    • TRPM-2
    • TRPM2
    • TRPM2B
    • Trpmb
    see all
  • 功能
    Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
  • 组织特异性
    Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.
  • 序列相似性
    Belongs to the clusterin family.
  • 翻译后修饰
    Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
    Polyubiquitinated, leading to proteasomal degradation.
    Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.
  • 细胞定位
    Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis.
  • Information by UniProt

文献

ab109150 has not yet been referenced specifically in any publications.

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