Recombinant人CLIC4 protein (ab104744)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 序列
      MGSSHHHHHHSSGLVPRGSHMALSMPLNGLKEEDKEPLIELFVKAGSDGE SIGNCPFSQRLFMILWLKGVVFSVTTVDLKRKPADLQNLAPGTHPPFITF NSEVKTDVNKIEEFLEEVLCPPKYLKLSPKHPESNTAGMDIFAKFSAYIK NSRPEANEALERGLLKTLQKLDEYLNSPLPDEIDENSMEDIKFSTRKFLD GNEMTLADCNLLPKLHIVKVVAKKYRNFDIPKEMTGIWRYLTNAYSRDEF TNTCPSDKEVEIAYSDVAKRLTK
    • 分子量
      31 kDa including tags
    • 氨基酸
      1 to 253
    • 标签
      His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab104744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Mass Spectrometry

    SDS-PAGE

  • 质谱法
    MALDI-TOF
  • 纯度
    > 95 % SDS-PAGE.
    ab104744 is purified using conventional chromatography techniques.
  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0

常规信息

  • 别名
    • Chloride intracellular channel 4
    • Chloride intracellular channel 4 (mitochondrial)
    • Chloride intracellular channel 4 like
    • Chloride intracellular channel protein 4
    • Clic4
    • CLIC4_HUMAN
    • CLIC4L
    • DKFZP566G223
    • FLJ38640
    • H1
    • HUH1
    • Intracellular chloride ion channel protein p64H1
    • MC3S5
    • mtCLIC
    • p64H1
    see all
  • 功能
    Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).
  • 组织特异性
    Detected in epithelial cells from colon, esophagus and kidney (at protein level). Expression is prominent in heart, kidney, placenta and skeletal muscle.
  • 序列相似性
    Belongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • 结构域
    Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • 细胞定位
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic vesicle membrane. Nucleus matrix. Cell membrane. Mitochondrion. Cell junction. Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles.
  • Information by UniProt

图片

  • 15% SDS-PAGE (3µg).

文献

ab104744 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Used this purified protein as a positive control to determine antibody specificity to CLIC4 in western blots. Very low amount (0.5 ug/lane) of protein was sufficient to yield a positive result. Purified CLIC1 protein (ab95486) from Abcam was used as a negative control.
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提交于 Jun 16 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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