Recombinant人Claspin protein (ab125977)

概述

  • 产品名称Recombinant人Claspin protein
  • 蛋白长度Protein fragment

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionQ9HAW4
    • 种属Human
    • 分子量24 kDa including tags
    • 氨基酸1126 to 1330

技术指标

Our Abpromise guarantee covers the use of ab125977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at -20°C.

    Constituents: 0.32% Tris HCl, 0.58% Sodium chloride

  • 复溶Reconstitute with water to desired concentration

常规信息

  • 别名
    • Claspin
    • Claspin homolog
    • claspin homolog (Xenopus laevis)
    • CLSPN
    • CLSPN protein
    • CLSPN_HUMAN
    • hClaspin
    • Hu Claspin
    see all
  • 功能Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.
  • 序列相似性Belongs to the claspin family.
  • 结构域The C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.
  • 翻译后修饰Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.
  • 细胞定位Nucleus.
  • Information by UniProt

Recombinant Human Claspin protein (ab125977)参考文献

ab125977 has not yet been referenced specifically in any publications.

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