Recombinant人CKII alpha prime polypeptide protein (ab90035)



  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human


Our Abpromise guarantee covers the use of ab90035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Activity: > 100.000 U/mg (1 Unit is defined as 1 picomole phosphate transferred to synthetic peptide RRRDDDSDDD per min at 37oC).
  • 应用

    Functional Studies


  • 纯度> 95 % SDS-PAGE.
    Purity is > 99% by SDS-PAGE.
  • 形式Liquid
  • Concentration information loading...


  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 200mM Sodium chloride, 20mM Tris HCl, 2mM DTT, 1mM EDTA, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.


  • 别名
    • Casein kinase 2 alpha prime polypeptide
    • Casein kinase II alpha' chain
    • Casein kinase II subunit alpha'
    • CK II
    • CK II alpha'
    • CK2A2
    • CKII
    • CSK22_HUMAN
    • CSNK2A1
    • CSNK2A2
    • FLJ43934
    see all
  • 功能Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV.
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt

Recombinant human CKII alpha prime polypeptide protein (ab90035)参考文献

ab90035 has not yet been referenced specifically in any publications.

Product Wall

The endogenous enzyme is a heterotetramer consisting of 2α/2β subunits. However, I can confirm that ab90035 consists of just the α' subunit of CK2. It's molecular weight of 84.3 kDa is due to a N-MBP fusion tag.

We did not p...

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