Recombinant人CKII alpha prime polypeptide protein (ab105125)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • AccessionP19784
    • 种属Human
    • 分子量64 kDa including tags
    • 氨基酸1 to 350

技术指标

Our Abpromise guarantee covers the use of ab105125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity: 63 nmol/min/mg
  • 应用

    Western blot

    Functional Studies

  • 纯度> 95 % Densitometry.

  • 形式Liquid
  • 补充说明

    ab204855 (CKII peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Casein kinase 2 alpha prime polypeptide
    • Casein kinase II alpha' chain
    • Casein kinase II subunit alpha'
    • CK II
    • CK II alpha'
    • CK2A2
    • CKII
    • CSK22_HUMAN
    • CSNK2A1
    • CSNK2A2
    • FLJ43934
    see all
  • 功能Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV.
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt

Recombinant human CKII alpha prime polypeptide protein 图像

  • Sample Kinase Activity Plot. The specific activity of CKII alpha prime polypeptide was determined to be 63 nmol/min/mg.
  • Recombinant human CKII alpha prime polypeptide protein (ab105125) + Full length recombinant human CKII alpha prime polypeptide plus proprietary tag

Recombinant human CKII alpha prime polypeptide protein (ab105125)参考文献

ab105125 has not yet been referenced specifically in any publications.

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