Recombinant人CK1 epsilon protein (ab104024)

概述

  • 产品名称Recombinant人CK1 epsilon protein
  • 蛋白长度Full length protein

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • AccessionP49674
    • 种属Human
    • 分子量72 kDa including tags
    • 氨基酸1 to 416

技术指标

Our Abpromise guarantee covers the use of ab104024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The Specific activity of ab104024 was determined to be 46 nmol/min/mg.
  • 应用

    Functional Studies

    SDS-PAGE

  • 纯度> 75 % SDS-PAGE.
    Purity was determined to be >75% by densitometry.
  • 形式Liquid
  • 补充说明

    ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Casein kinase 1 epsilon
    • Casein kinase I
    • Casein kinase I epsilon
    • Casein kinase I isoform epsilon
    • CKI epsilon
    • CKI-epsilon
    • CKIe
    • CKIepsilon
    • CSNK 1E
    • CSNK1E
    • DBT
    • Doubletime, Drosophila, homolog of
    • EC 2.7.11.1
    • epsilon isoform
    • HCK IE
    • KC1E
    • KC1E_HUMAN
    • kinase CK1 epsilon
    • MGC 10398
    see all
  • 功能Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. Inhibits cytokine-induced granuloytic differentiation.
  • 组织特异性Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.
  • 序列相似性Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt

Recombinant human CK1 epsilon protein 图像

  • The Specific activity of ab104024 was determined to be 46 nmol/min/mg.
  • SDS-PAGE showing ab104024 at approximately 72kDa.

Recombinant human CK1 epsilon protein (ab104024)参考文献

ab104024 has not yet been referenced specifically in any publications.

Product Wall

Please see the requested protocol below. Please contact me again if you have any further questions.

====

Kinase assay protocol for CK1-epsilon, Abcam ab104024

Thaw [32P]-ATP assay cocktail in a designated radioactive worki...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"