Recombinant人CHMP2B protein (ab109959)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionQ9UQN3
    • 种属Human
    • 序列MGSSHHHHHHSSGLVPRGSHMASLFKKKTVDDVIKEQNRELRGTQRAIIR DRAALEKQEKQLELEIKKMAKIGNKEACKVLAKQLVHLRKQKTRTFAVSS KVTSMSTQTKVMNSQMKMAGAMSTTAKTMQAVNKKMDPQKTLQTMQNFQK ENMKMEMTEEMINDTLDDIFDGSDDEEESQDIVNQVLDEIGIEISGKMAK APSAARSLPSASTSKATISDEEIERQLKALGVD
    • 分子量26 kDa including tags
    • 氨基酸1 to 213
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab109959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Mass Spectrometry

  • 质谱法
    MALDI-TOF
  • 纯度> 90 % SDS-PAGE.
    ab109959 was purified using conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 10% Glycerol, 0.32% Tris HCl, 0.02% DTT, 0.58% Sodium chloride

常规信息

  • 别名
    • ALS17
    • Charged multivesicular body protein 2b
    • CHM2B_HUMAN
    • CHMP family, member 2B
    • CHMP2.5
    • CHMP2b
    • Chromatin modifying protein 2b
    • Chromatin-modifying protein 2b
    • DMT1
    • hVps2-2
    • Vacuolar protein sorting 2, yeast, homolog of, B
    • Vacuolar protein sorting 2-2
    • Vacuolar protein sorting-associated protein 2-2
    • VPS2 homolog B
    • Vps2-2
    • VPS2B
    see all
  • 功能Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4.
  • 组织特异性Widely expressed. Expressed in brain, heart, skeletal muscle, spleen, kidney, liver, small intestine, pancreas, lung, placenta and leukocytes. In brain, it is expressed in cerebellum, cerebral cortex, medulla, spinal chord, occipital lobe, frontal lobe, temporal lobe and putamen.
  • 疾病相关Defects in CHMP2B are the cause of frontotemporal dementia, chromosome 3-linked (FTD3) [MIM:600795]. FTD3 is characterized by an onset of dementia in the late 50's initially characterized by behavioral and personality changes including apathy, restlessness, disinhibition and hyperorality, progressing to stereotyped behaviors, non-fluent aphasia, mutism and dystonia, with a marked lack of insight. The brains of individuals with FTD3 have no distinctive neuropathological features. They show global cortical and central atrophy, but no beta-amyloid deposits.
  • 序列相似性Belongs to the SNF7 family.
  • 结构域The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
  • 细胞定位Cytoplasm > cytosol. Late endosome membrane.
  • Information by UniProt

Recombinant Human CHMP2B protein 图像

  • 15% SDS-PAGE analysis of 3 µg ab109959.

Recombinant Human CHMP2B protein (ab109959)参考文献

ab109959 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab109959.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"