Recombinant人Chk2 protein (ab42604)



  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human


Our Abpromise guarantee covers the use of ab42604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性1 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of CHKtide substrate per minute at pH 7.4 and 30C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.1 mM ATP.
  • 应用

    Inhibition Assay

  • 形式Liquid
  • Concentration information loading...


  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.


  • 别名
    • CDS 1
    • Cds1
    • Cds1 homolog
    • Checkpoint kinase 2
    • Checkpoint like protein CHK2
    • CHEK 2
    • Chek2
    • Chk 2
    • CHK2 checkpoint homolog
    • CHK2 checkpoint homolog (S. pombe)
    • CHK2_HUMAN
    • hCds1
    • HuCds 1
    • LFS 2
    • LFS2
    • PP1425
    • RAD 53
    • RAD53
    • Rad53 homolog
    • Serine/threonine protein kinase Chk2
    • Serine/threonine-protein kinase Chk2
    see all
  • 功能Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
  • 组织特异性High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
  • 疾病相关Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
    Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
    Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
    Contains 1 FHA domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylated by PLK4.
  • 细胞定位Nucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
  • Information by UniProt

Recombinant human Chk2 protein (ab42604)参考文献

ab42604 has not yet been referenced specifically in any publications.

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