概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human
    • 分子量17 kDa
    • 额外的序列信息ENPA Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 17,015 Dalton.

技术指标

Our Abpromise guarantee covers the use of ab73635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性ab73635 reacts with Scleroderma patient sera.
  • 应用

    Other

    ELISA

    Western blot

  • 纯度> 90 % SDS-PAGE.
    ab73635 is purified by proprietary chromatographic techniques.
  • 形式Liquid
  • 补充说明ab73635 reacts with Scleroderma patient sera.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 6M Urea, 20mM HEPES, 100mM Sodium chloride, pH 8

常规信息

  • 别名
    • CENP A
    • CENP-A
    • cenpa
    • CENPA_HUMAN
    • Centromere autoantigen A
    • Centromere protein A
    • Centromere protein A 17kDa
    • Histone H3 like centromeric protein A
    • Histone H3-like centromeric protein A
    see all
  • 功能Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
  • 序列相似性Belongs to the histone H3 family.
  • 结构域The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containing CENPA and is necessary and sufficient to mediate the localization into centromeres.
  • 翻译后修饰Ubiquitinated (Probable). Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway.
    Phosphorylation of Ser-7 by Aurora-A/STK6 and Aurora-B/STK12 during prophase is required for localization of Aurora-A/STK6 and Aurora-B/STK12 at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by Aurora-A/STK6 and is maintained by Aurora-B/STK12.
  • 细胞定位Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height.
  • Information by UniProt

Recombinant Human CENPA protein (ab73635)参考文献

This product has been referenced in:
  • Favoino E  et al. Autoantibodies recognizing the amino terminal 1-17 segment of CENP-A display unique specificities in systemic sclerosis. PLoS One 8:e61453 (2013). WB . Read more (PubMed: 23613856) »

See 1 Publication for this product

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