Recombinant人CDK7 + Cyclin H + MNAT1 protein (ab64303)

概述

  • 产品名称Recombinant人CDK7 + Cyclin H + MNAT1 protein
  • 蛋白长度Full length protein

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human

相关产品

技术指标

Our Abpromise guarantee covers the use of ab64303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity: 19 nmol/min/mg.
  • 应用

    SDS-PAGE

    Functional Studies

  • 纯度> 90 % SDS-PAGE.

  • 形式Liquid
  • 补充说明

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 150mM Imidazole
    Constituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 0.2mM DTT, 0.1mM PMSF, pH 7

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • CAK
    • CAK1
    • CCNH
    • CDK activating kinase
    • Cyclin dependent kinase 7
    • Cyclin H
    • MAT1
    • MO15
    • MO15 associated protein
    • p34
    • p35
    • p36
    • p37
    • STK1
    see all
  • 相关性CDK7: Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle. Cyclin H: Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle. MNAT1: Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.
  • 细胞定位Nuclear

Recombinant human CDK7 + Cyclin H + MNAT1 protein 图像

  • The specific activity of CDK7 + Cyclin H + MNAT1 (ab64303) was determined to be 19nmol/min/mg as per activity assay protocol.

  • SDS-PAGE analysis of ab64303. The purity of CDK7 + Cyclin H + MNAT1 (ab64303) was determined to be >90% by densitometry, CDK7 approx. MW 40kDa, CyclinH1 approx. MW 39kDa and MNAT1  approx. MW 37kDa

Recombinant human CDK7 + Cyclin H + MNAT1 protein (ab64303)参考文献

ab64303 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"