Recombinant人CDK6 + CCND3 protein (ab84557)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab84557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity of ab84557 was determined to be 36 nmol/min/mg.
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 形式Liquid
  • 补充说明

    ab56270 (Human Rb protein fragment) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 150mM Imidazole
    Constituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 0.2mM DTT, 0.1mM PMSF, pH 7.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • CCND 3
    • CCND3
    • CDK 6
    • CDK6
    • Cell division protein kinase 6
    • Crk 2
    • Crk2
    • Cyclin D3
    • Cyclin dependent kinase 6
    • CyclinD3
    • D3 type cyclin
    • G1 S specific cyclin D3
    • G1/S specific cyclin D3
    • MGC59692
    • p40
    • PLSTIRE
    • Serine/threonine protein kinase PLSTIRE
    • STQTL11
    see all
  • 相关性CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of Cdk6 first appears in mid-G1 phase, and is controlled by regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Cyclin D3 is a G1 cyclin closely related to cyclin D1 and D2. Cyclins D1, D2, and D3 are members of a growing superfamily of cyclins, proteins that govern transitions through distinct phases of the cell cycle by regulating the activity of cyclin-dependent kinases (cdks). All three known members of the D-type subfamily form active kinase complexes in vivo with a distinct subset of cdks, particularly with cdk4 and cdk6.
  • 细胞定位CCND3: Cytoplasm. Nucleus

Recombinant human CDK6 + CCND3 protein 图像

  • SDS-PAGE of ab84557. CDK6 approximate MW 40kDa and CCND3 approximate MW 35kDa.
  • Kinase Assay: Specific activity of CDK6/CCND3 was determined to be 36 nmol/min/mg using protein substrate Rb.

Recombinant human CDK6 + CCND3 protein (ab84557)参考文献

ab84557 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"