重组人CDK5R1(p25)蛋白. (ab125653)

概述

  • 产品名称
    重组人CDK5R1(p25)蛋白.
  • 蛋白长度
    Full length protein
  • 描述
    重组人CDK5R1蛋白

描述

  • 性质
    Recombinant
  • 来源
    Baculovirus infected Sf9 cells
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 分子量
      49 kDa including tags
    • 氨基酸
      98 to 307

技术指标

Our Abpromise guarantee covers the use of ab125653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    SDS-PAGE

  • 纯度
    > 85 % Densitometry.
    Purity was determined to be >85% by densitometry.
  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

常规信息

  • 别名
    • CD5R1_HUMAN
    • CDK5 activator 1
    • CDK5R1
    • Cyclin-dependent kinase 5 activator 1
    • Cyclin-dependent kinase 5 regulatory subunit 1
    • p23
    • p25
    • p35
    • Tau protein kinase II 23 kDa subunit
    • TPKII regulatory subunit
    see all
  • 功能
    p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Activator of TPKII.
  • 组织特异性
    Brain and neuron specific.
  • 序列相似性
    Belongs to the cyclin-dependent kinase 5 activator family.
  • 翻译后修饰
    The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo.
    Probably myristoylated. The Gly-2-Ala mutant is absent of the cell periphery, suggesting that a proper myristoylation signal is essential for the proper distribution of p35.
  • 细胞定位
    Cell membrane. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals and Nucleus. Cytoplasm > perinuclear region. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites.
  • Information by UniProt

图片

  • SDS-PAGE analysis of ab125653.

文献

ab125653 has not yet been referenced specifically in any publications.

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