重组人CDK5R1(p25)蛋白. (ab125653)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Tags: GST tag N-Terminus
- Suitable for: WB, SDS-PAGE
描述
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产品名称
重组人CDK5R1(p25)蛋白. -
纯度
> 85 % Densitometry.
Purity was determined to be >85% by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
49 kDa including tags -
氨基酸
98 to 307 -
标签
GST tag N-Terminus
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描述
重组人CDK5R1蛋白
技术指标
Our Abpromise guarantee covers the use of ab125653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
常规信息
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别名
- CD5R1_HUMAN
- CDK5 activator 1
- CDK5R1
see all -
功能
p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Activator of TPKII. -
组织特异性
Brain and neuron specific. -
序列相似性
Belongs to the cyclin-dependent kinase 5 activator family. -
翻译后修饰
The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo.
Probably myristoylated. The Gly-2-Ala mutant is absent of the cell periphery, suggesting that a proper myristoylation signal is essential for the proper distribution of p35. -
细胞定位
Cell membrane. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals and Nucleus. Cytoplasm > perinuclear region. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab125653 被引用在 1 文献中.
- Ding Y et al. Role of cyclin-dependent kinase 5 in early brain injury following experimental subarachnoid hemorrhage. Exp Ther Med 23:147 (2022). PubMed: 35069828