Recombinant人Cdk4 + Cyclin D1 protein (ab55695)

概述

  • 产品名称Recombinant人Cdk4 + Cyclin D1 protein
  • 蛋白长度Full length protein

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab55695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性14nmol/min/mg using protein substrate Rb (773-928).
  • 应用

    SDS-PAGE

    Functional Studies

  • 形式Liquid
  • 补充说明 14nmol/min/mg using protein substrate Rb (773-928).

    ab56270 (Human Rb protein fragment) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

常规信息

  • 别名
    • B cell CLL lymphoma 1
    • BCL 1 oncogene
    • BCL1
    • Cell division protein kinase 4
    • CMM3
    • Cyclin dependent kinase 4
    • PRAD1
    • PSK J3
    see all
  • 相关性CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase. Phosphorylation at Thr-172 is necessary for enzymatic activity. CDK4 mutations are involved in tumor formation, with defects in CDK4 resulting in cutaneous malignant melanoma 3 (CMM3). Cyclin D1 is essential for the control of the cell cycle at the G1/S transition. It interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex.
  • 细胞定位Nuclear

Recombinant Human Cdk4 + Cyclin D1 protein 图像

  • Kinase assay using ab55695 using protein substrate Rb. The specific activity of CDK4/CyclinD1 is 14 nmol/min/mg.
  • SDS-PAGE analysis of ab55695 with molecular weight markers. Approximate molecular weights: 57kDa (CDK4)/61kDa (CyclinD1).

Recombinant Human Cdk4 + Cyclin D1 protein (ab55695)参考文献

This product has been referenced in:
  • Kondo E  et al. Tumour lineage-homing cell-penetrating peptides as anticancer molecular delivery systems. Nat Commun 3:951 (2012). Read more (PubMed: 22805558) »
  • Nakajima K  et al. Coordinated regulation of differentiation and proliferation of embryonic cardiomyocytes by a jumonji (Jarid2)-cyclin D1 pathway. Development 138:1771-82 (2011). Read more (PubMed: 21447557) »

See all 2 Publications for this product

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