Recombinant人CDK4 + CCND3 protein (ab85646)

概述

  • 产品名称Recombinant人CDK4 + CCND3 protein
  • 蛋白长度Full length protein

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab85646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The Specific activity of ab85646 was determined to be 16 nmol/min/mg.
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 形式Liquid
  • 补充说明

    ab56270 (Human Rb protein fragment) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • CCND 3
    • CCND3
    • Cdk 4
    • CDK4 protein
    • Cell division kinase 4
    • Cell division protein kinase 4
    • CMM 3
    • CMM3
    • Crk3
    • Cyclin D3
    • Cyclin dependent kinase 4
    • CyclinD3
    • D3 type cyclin
    • G1 S specific cyclin D3
    • G1/S specific cyclin D3
    • Melanoma cutaneous malignant 3
    • MGC14458
    • p34 cdk4
    • PSK J3
    see all
  • 相关性CDK4 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. CDK4 is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1/S phase, which is controlled by the regulatory subunits D type cyclins and CDK inhibitor p16(INK4a). CDK4 was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as its related proteins including D type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. CCND3 (Cyclin D3) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. CCND3 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. It has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb.
  • 细胞定位CCND3: Cytoplasm. Nucleus.

Recombinant human CDK4 + CCND3 protein 图像

  • The Specific activity of ab85646 was determined to be 16 nmol/min/mg.
  • SDS-PAGE showing ab85646 at approximately 58kDa (both proteins).

Recombinant human CDK4 + CCND3 protein (ab85646)参考文献

ab85646 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"