重组人CDK2 + CCNA1蛋白(ab105205)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, WB, Functional Studies
描述
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产品名称
重组人CDK2 + CCNA1蛋白 -
生物活性
The Specific activity of ab105205 was determined to be 83 nmol/min/mg.
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纯度
> 85 % SDS-PAGE.
Purity was determined to be >85% by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
额外的序列信息
The CDK2 gene accession number is NM_001798; CyclinA1 is NM_003914.
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技术指标
Our Abpromise guarantee covers the use of ab105205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Western blot
Functional Studies
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- CCN A1
- CCNA 1
- CCNA1
see all -
相关性
CDK2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. Cyclin A1 (CCNA1) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin A1 is expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. It binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. Cyclin A1 was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. -
细胞定位
CCNA1: Nuclear
图片
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The specific activity of CDK2 + CCNA1 (ab105205) was determined to be 74 nmol/min/mg as per activity assay protocol
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SDS PAGE analysis of ab105205
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SDS PAGE analysis of ab105205
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The Specific activity of ab105205 was determined to be 83 nmol/min/mg.
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SDS-PAGE showing ab105205 at approximately 58 and 81kDa respectively.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab105205 尚未被引用在任何文献中。