Recombinant人Cdc34 protein (ab58720)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab58720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Functional Studies

  • 纯度> 95 % SDS-PAGE.

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 50mM Sodium chloride, 50mM HEPES, 1mM DTT, pH 8.0

常规信息

  • 别名
    • Cdc 34
    • Cdc34
    • Cell division cycle 34
    • Cell division cycle 34 homolog
    • Cell division cycle 34 homolog (S. cerevisiae)
    • E2 CDC34
    • UB2R1_HUMAN
    • UBC 3
    • UBC3
    • UBCH3
    • UBE2 R1
    • UBE2R1
    • Ubiquitin carrier protein
    • Ubiquitin conjugating enzyme Cdc34
    • Ubiquitin conjugating enzyme E2 32 kDa complementing
    • Ubiquitin protein ligase
    • Ubiquitin protein ligase R1
    • Ubiquitin-conjugating enzyme E2 R1
    • Ubiquitin-conjugating enzyme E2-32 kDa complementing
    • Ubiquitin-conjugating enzyme E2-CDC34
    • Ubiquitin-protein ligase R1
    see all
  • 功能Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase throught its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes.
  • 组织特异性Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the ubiquitin-conjugating enzyme family.
  • 结构域The C-terminal acidic tail is required for nuclear localization and is involved in the binding to SCF E3 ligase complexes, and more specifically with the CUL1 subunit.
  • 翻译后修饰Autoubiquitinated. Autoubiquitination is promoted by the human herpes virus 1 protein ICP0 and leads to degradation by the Ubiquitin-proteasomal pathway.
    Phosphorylated by CK2. Phosphorylation of the C-terminal tail by CK2 controles the nuclear localization.
  • 细胞定位Cytoplasm. Nucleus. The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase.
  • Information by UniProt

Recombinant Human Cdc34 protein (ab58720)参考文献

ab58720 has not yet been referenced specifically in any publications.

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