Recombinant人Cdc20 protein (ab114348)

概述

描述

  • 性质Recombinant
  • 来源Wheat germ
  • 氨基酸序列
    • AccessionQ12834
    • 种属Human
    • 序列MAQFAFESDLHSLLQLDAPIPNAPPARWQRKAKEAAGPAPSPMRAANRSH SAGRTPGRTPGKSSSKVQTTPSKPGGDRYIPHRSAAQMEVASFLLSKENQ
    • 分子量37 kDa including tags
    • 氨基酸1 to 100

技术指标

Our Abpromise guarantee covers the use of ab114348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    ELISA

    SDS-PAGE

    Western blot

  • 形式Liquid
  • 补充说明Protein concentration is above or equal to 0.05 mg/ml.
    Best use within three months from the date of receipt of this protein.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

常规信息

  • 别名
    • bA276H19.3
    • Cdc 20
    • CDC20
    • CDC20 cell division cycle 20 homolog
    • CDC20_HUMAN
    • CDC20A
    • Cell division cycle 20
    • Cell division cycle 20 homolog
    • Cell division cycle 20 homolog (S. cerevisiae)
    • Cell division cycle protein 20 homolog
    • fizzy
    • MGC102824
    • p55CDC
    see all
  • 功能Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the WD repeat CDC20/Fizzy family.
    Contains 7 WD repeats.
  • 发展阶段Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.
  • 翻译后修饰Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.
    Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C.
  • Information by UniProt

Recombinant Human Cdc20 protein 图像

  • ab114348 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

Recombinant Human Cdc20 protein (ab114348)参考文献

ab114348 has not yet been referenced specifically in any publications.

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