Recombinant人CD45 protein (ab94517)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human
    • 氨基酸604 to 1304

技术指标

Our Abpromise guarantee covers the use of ab94517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The specific activity of ab94517 is 11,500 nmol phosphate released /min/mg
  • 应用

    Functional Studies

    Western blot

    SDS-PAGE

  • 纯度> 90 % SDS-PAGE.
    >90% as determined by densitometry
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 150mM Imidazole
    Constituents: 25% Glycerol, 50mM MOPS, 300mM Sodium chloride, 0.25mM DTT, 0.1mM PMSF, pH 7.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • B220
    • CD 45
    • CD45
    • CD45 antigen
    • CD45R
    • GP180
    • L-CA
    • LCA
    • Leukocyte common antigen
    • loc
    • Ly-5
    • LY5
    • Ly5, homolog of
    • Lyt-4
    • OTTHUMP00000033813
    • OTTHUMP00000033816
    • OTTHUMP00000033817
    • OTTHUMP00000038574
    • Protein tyrosine phosphatase receptor type c polypeptide
    • protein tyrosine phosphatase, receptor type, C
    • Ptprc
    • PTPRC_HUMAN
    • Receptor-type tyrosine-protein phosphatase C
    • T200
    • T200 glycoprotein
    • T200 leukocyte common antigen
    see all
  • 功能Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
  • 疾病相关Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • 序列相似性Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • 结构域The first PTPase domain interacts with SKAP1.
  • 翻译后修饰Heavily N- and O-glycosylated.
  • 细胞定位Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt

Recombinant human CD45 protein 图像

  • ab94517 in a Sample Kinase Activity Plot.
  • ab94517 analysed by SDS PAGE at approximately 95kDa

Recombinant human CD45 protein (ab94517)参考文献

ab94517 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"