Recombinant人CD40L protein (Soluble) (ab51956)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP29965
    • 种属Human
    • 序列MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLT VKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTH SSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL
    • 分子量16 kDa
    • 氨基酸113 to 261
    • 额外的序列信息Full length soluble form.

技术指标

Our Abpromise guarantee covers the use of ab51956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Biological Activity: Determined by the stimulation of IL-12 induction by human peripheral blood mononuclear cells (PBMC) and the stimulation of IL-8 induction by human PBMC. The expected ED50 for this effect is 5-10 ng/ml.Note: Results may vary with PBMC donors.
  • 应用

    SDS-PAGE

    Functional Studies

  • 纯度> 95 % SDS-PAGE.
    >98% by SDS-PAGE and HPLC analyses
  • 形式Lyophilised
  • 补充说明The reconstituted solution can be diluted into aqueous buffers or stored at 4°C for 1 week or –20°C for future use.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Lyophilized from 10mM Sodium phosphate, pH 7.5
    Endotoxin level is <0.1 ng per µg of CD40L

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Reconstitute in 5-10 mM Sodium Phosphate (pH 7.5) to a concentration of 0.1-1.0 mg/ml.

常规信息

  • 别名
    • CD 40L
    • CD154
    • CD40 antigen ligand
    • CD40 ligand
    • CD40 ligand, soluble form
    • CD40-L
    • CD40L
    • CD40L_HUMAN
    • CD40LG
    • gp39
    • hCD40L
    • HIGM1
    • IGM
    • IMD3
    • T B cell activating molecule
    • T BAM
    • T-cell antigen Gp39
    • TNF-related activation protein
    • TNFSF5
    • TrAP
    • Tumor necrosis factor (ligand) superfamily member 5
    • Tumor necrosis factor ligand superfamily member 5
    see all
  • 功能Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
    Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
  • 组织特异性Specifically expressed on activated CD4+ T-lymphocytes.
  • 疾病相关Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
  • 序列相似性Belongs to the tumor necrosis factor family.
  • 翻译后修饰The soluble form derives from the membrane form by proteolytic processing.
    N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
    Not O-glycosylated.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt

Recombinant human CD40L protein (Soluble) (ab51956)参考文献

ab51956 has not yet been referenced specifically in any publications.

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