重组人Caspase-4蛋白(Active) (ab51994)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies
描述
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产品名称
重组人Caspase-4蛋白(Active) -
生物活性
One unit of the recombinant caspase-4 is the enzyme activity that cleaves 1 nmol of the caspase substrate WEHD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol and 10 mM DTT.
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纯度
> 95 % SDS-PAGE. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
标签
His tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab51994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
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形式
Lyophilized -
补充说明
This product is manufactured by BioVision, an Abcam company and was previously called 1084 Caspase-4, human recombinant. 1084-100 is the same size as the 100 unit size of ab51994.
This protein is a heterotetramer consisting of two large and two small subunits. Active Caspase 4 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays.
5000 units/mg.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 15% Glycerol (glycerin, glycerine), PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute to 1 unit per µl in PBS containing 15% glycerol. Aliquot and store immediately at -80°C.
常规信息
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别名
- Apoptotic cysteine protease Mih1/TX
- CASP-4
- CASP4
see all -
功能
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1. -
组织特异性
Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain. -
序列相似性
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
翻译后修饰
The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab51994 被引用在 3 文献中.
- Amara N et al. Selective activation of PFKL suppresses the phagocytic oxidative burst. Cell 184:4480-4494.e15 (2021). PubMed: 34320407
- Chen Q et al. Nitrosonisoldipine is a selective inhibitor of inflammatory caspases and protects against pyroptosis and related septic shock. Eur J Immunol 51:1234-1245 (2021). PubMed: 33454984
- Sollberger G et al. Gasdermin D plays a vital role in the generation of neutrophil extracellular traps. Sci Immunol 3:N/A (2018). PubMed: 30143555