Recombinant人Caspase 1 protein (ab39901)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列NPAMPTSSGSEGNVKLCSLEEAQRIWKQKSAEIYPIMDKSSRTRLALIIC NEEFDSIPRRTGAEVDITGMTMLLQNLGYSVDVKKNLTASDMTTELEAFA HRPEHKTSDSTFLVFMSHGIREGICGKKHSEQVPDILQLNAIFNMLNTKN CPSLKDKPKVIIIQACRGDSPGVVWFKDSVGVSGNLSLPTTEEFEDDAIK KAHIEKDFIAFCSSTPDNVSWRHPTMGSVFIGRLIEHMQEYACSCDVEEI FRKVRFSFEQPDGRAQMPTTERVTLTRCFYLFPGH

技术指标

Our Abpromise guarantee covers the use of ab39901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Activity : >5000 units/mg
  • 应用

    Functional Studies

  • 形式Lyophilised
  • 补充说明

    Active caspase-1 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. The active caspase 1 preferentially cleaves caspase-1 substrates (e.g., YVAD-AFC or YVAD-pNA)

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Reconstitute to 1 unit per µl in PBS containing 15% glycerol

常规信息

  • 别名
    • CASP1
    • CASP1 nirs variant 1
    • Caspase 1 apoptosis related cysteine peptidase
    • caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
    • ICE
    • ICE-like protease
    • IL1 B convertase
    • IL1 beta converting enzyme
    • IL1BC
    • IL1BCE
    • Interleukin 1 B converting enzyme
    • Interleukin 1 beta convertase
    • P45
    see all
  • 相关性Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase 1 is similar to the cell death gene CED3 of C. elegans and regulates multiple proinflammatory cytokines, including Interleukin 1b and interferon-gamma-inducing factor. Caspase 1 plays a role in down stream of Caspase 8 which is involved in Fas-mediated apoptosis.
  • 细胞定位Cytoplasmic

Recombinant human Caspase 1 protein (ab39901)参考文献

ab39901 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

I certainly understand that the mass concentration would be imperative when using in Western blot. Unfortunately this is considered proprietary information and I cannot provide it.
I've looked through our catalog...

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Thank you for your call earlier this week and for your patience while I have been in touch with the producing lab about ab39901.

This protein is provided as the heterotetramer of the small (10 kDa) and large (20 kDa) subunits of caspase 1. It...

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Thank you for contacting Abcam.

For ab39901, Caspase 1, we recommend using 1 unit/assay for analyzing caspase activity and this protein has a biological activity : >5000 units/mg and it is supplied lyophilized and it should be reconstitute...

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Thank you for your inquiry. Please see below for the unit definition for ab39901 (Caspase 1 protein (Active)): One unit of the recombinant Caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA (pNA: pnitroanaline) per h...

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Thank you for contacting us. I have heard back from the lab with the following information: The dilution buffer can be used to dilute the caspase 1 protein (ab39901) when being used as a positive control. Thus, the protocol is correct as it is. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"