重组人Caspase-1蛋白(Active) (ab39901)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人Caspase-1蛋白(Active) -
生物活性
Specific Activity: > 5000 units/mg. Unit definition: One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
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纯度
> 80 % SDS-PAGE.
Affinity chromatography. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
NPAMPTSSGS EGNVKLCSLE EAQRIWKQKS AEIYPIMDKS SRTRLALIIC NEEFDSIPRR TGAEVDITGM TMLLQNLGYS VDVKKNLTAS DMTTELEAFA HRPEHKTSDS TFLVFMSHGI REGICGKKHS EQVPDILQLN AIFNMLNTKN CPSLKDKPKV IIIQACRGDS PGVVWFKDSV GVSGNLSLPT TEEFEDDAIK KAHIEKDFIA FCSSTPDNVS WRHPTMGSVF IGRLIEHMQE YACSCDVEEI FRKVRFSFEQ PDGRAQMPTT ERVTLTRCFY LFPGH -
预测分子量
32 kDa -
分子量信息
Large subunit 20 kDa and small subunit 10 kDa. -
氨基酸
120 to 404 -
标签
His tag N-Terminus -
额外的序列信息
(Gene ID 834) (NP_150634.1) Full length mature protein, minus propeptide (aa 1-119).
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技术指标
Our Abpromise guarantee covers the use of ab39901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Lyophilized -
补充说明
This product is manufactured by BioVision, an Abcam company and was previously called 1081 Active Caspase-1, Human Recombinant. 1081-25 is the same size as the 25 unit size of ab39901.
Active caspase-1 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays.
The active caspase 1 preferentially cleaves caspase-1 substrates (e.g., YVAD-AFC or YVAD-pNA).
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 10.269% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphateThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- CASP-1
- CASP1
- CASP1 nirs variant 1
see all -
相关性
Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase 1 is similar to the cell death gene CED3 of C. elegans and regulates multiple proinflammatory cytokines, including Interleukin 1b and interferon-gamma-inducing factor. Caspase 1 plays a role in down stream of Caspase 8 which is involved in Fas-mediated apoptosis. -
细胞定位
Cytoplasmic
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (5)
ab39901 被引用在 5 文献中.
- Planès R et al. Human NLRP1 is a sensor of pathogenic coronavirus 3CL proteases in lung epithelial cells. Mol Cell 82:2385-2400.e9 (2022). PubMed: 35594856
- Iban-Arias R et al. Role of Polyphenol-Derived Phenolic Acid in Mitigation of Inflammasome-Mediated Anxiety and Depression. Biomedicines 10:N/A (2022). PubMed: 35740286
- Qu D et al. Effects of Tranilast on Inflammasome and Macrophage Phenotype in a Mouse Model of Myocardial Infarction. J Interferon Cytokine Res 41:102-110 (2021). PubMed: 33750216
- De Witte C et al. Characterization of the non-glandular gastric region microbiota in Helicobacter suis-infected versus non-infected pigs identifies a potential role for Fusobacterium gastrosuis in gastric ulceration. Vet Res 50:39 (2019). PubMed: 31126330
- Du M et al. Transgenic Mice Overexpressing Serum Retinol-Binding Protein Develop Progressive Retinal Degeneration through a Retinoid-Independent Mechanism. Mol Cell Biol 35:2771-89 (2015). Functional Studies . PubMed: 26055327