重组人Casein Kinase 1 gamma 2蛋白(ab185415)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Tags: His tag N-Terminus
- Suitable for: HPLC, SDS-PAGE
描述
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产品名称
重组人Casein Kinase 1 gamma 2蛋白
参阅全部 Casein Kinase 1 gamma 2 蛋白酶 -
纯度
> 95 % SDS-PAGE. -
内毒素水平
< 0.100 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MGSSHHHHHHSSGLVPRGSHMSKAGGGRSSHGIRSSGTSSGVLMVGPNFR VGKKIGCGNFGELRLGKNLYTNEYVAIKLEPIKSRAPQLHLEYRFYKQLS ATEGVPQVYYFGPCGKYNAMVLELLGPSLEDLFDLCDRTFTLKTVLMIAI QLITRMEYVHTKSLIYRDVKPENFLVGRPGTKRQHAIHIIDFGLAKEYID PETKKHIPYREHKSLTGTARYMSINTHLGKEQSRRDDLEALGHMFMYFLR GSLPWQGLKADTLKERYQKIGDTKRATPIEVLCENFPEEMATYLRYVRRL DFFEKPDYDYLRKLFTDLFDRSGFVFDYEYDWAGKPLPTPIGTVHTDLPS QPQLRDKTQPHSKNQALNSTNGELNADDPTAGHSNAPITAPAEVEVADET KCCCFFKRRKRKSLQRHK -
预测分子量
48 kDa including tags -
氨基酸
18 to 415 -
标签
His tag N-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab185415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
HPLC
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.32% Tris HCl, 2.9% Sodium chloride, 0.02% DTT, 10% Glycerol (glycerin, glycerine)
常规信息
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别名
- Casein kinase 1, gamma 2
- Casein kinase I isoform gamma 2
- Casein kinase I isoform gamma-2
see all -
功能
Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. -
组织特异性
Testis. -
序列相似性
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. -
细胞定位
Cytoplasm. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab185415 尚未被引用在任何文献中。