Recombinant人Casein Kinase 1 gamma 2 protein (ab104025)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • Accession P78368
    • 种属Human
    • 分子量73 kDa including tags
    • 氨基酸1 to 415

技术指标

Our Abpromise guarantee covers the use of ab104025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The Specific activity of ab104025 was determined to be 30 nmol/min/mg.
  • 应用

    Functional Studies

    Western blot

  • 纯度> 80 % SDS-PAGE.
    Purity was determined to be >85% by densitometry.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Casein kinase 1, gamma 2
    • Casein kinase I isoform gamma 2
    • Casein kinase I isoform gamma-2
    • CK1g2
    • CKI gamma 2
    • CKI-gamma 2
    • CSNK1G 2
    • Csnk1g2
    • KC1G2_HUMAN
    see all
  • 功能Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis.
  • 组织特异性Testis.
  • 序列相似性Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Cytoplasm.
  • Information by UniProt

Recombinant human Casein Kinase 1 gamma 2 protein 图像

  • The Specific activity of ab104025 was determined to be 30 nmol/min/mg.
  • SDS-PAGE showing ab104025 at approximately 73kDa.

Recombinant human Casein Kinase 1 gamma 2 protein (ab104025)参考文献

ab104025 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry. The only Casein Kinase 1 gamma 2 protein that we carry in the catalogue is ab104025, the protein you found. The concentration of this protein is 0.1 mg/ml. If you need to make a bulk order purchase (of 5 or more uni...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"