Recombinant人Casein Kinase 1 delta protein (ab103955)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • AccessionP48730
    • 种属Human
    • 分子量72 kDa including tags
    • 氨基酸1 to 415

技术指标

Our Abpromise guarantee covers the use of ab103955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The Specific activity of ab103955 was determined to be 82 nmol/min/mg.
  • 应用

    Functional Studies

    SDS-PAGE

    Western blot

  • 纯度> 80 % Densitometry.
    Purity was determined to be >80% by densitometry.
  • 形式Liquid
  • 补充说明

    ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Casein kinase I delta isoform
    • Casein kinase I isoform delta
    • CKI delta
    • CKI-delta
    • CKId
    • CKIdelta
    • CSNK 1D
    • CSNK1D
    • HCKID
    • KC1D_HUMAN
    • Protein kinase CK1 delta
    see all
  • 功能Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm.
  • 组织特异性Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. In blood, highly expressed in hemopoietic cells and mature granulocytes. Also found in monocytes and lymphocytes.
  • 疾病相关Defects in CSNK1D are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms.
  • 序列相似性Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated on serine and threonine residues.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt

Recombinant human Casein Kinase 1 delta protein 图像

  • The Specific activity of ab103955 was determined to be 82 nmol/min/mg.
  • SDS-PAGE showing ab103955 at approximately 72kDa.

Recombinant human Casein Kinase 1 delta protein (ab103955)参考文献

ab103955 has not yet been referenced specifically in any publications.

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