Recombinant人Casein Kinase 1 alpha protein (ab102102)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • AccessionP48729
    • 种属Human
    • 分子量62 kDa including tags
    • 氨基酸1 to 337

技术指标

Our Abpromise guarantee covers the use of ab102102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity is 120 nmol/min/mg
  • 应用

    Western blot

    Functional Studies

  • 纯度> 80 % Densitometry.
    The purity of Casein Kinase 1 alpha protein (Active) was >80% as assessed by densitometry.
  • 形式Liquid
  • 补充说明

    ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Casein kinase 1 alpha 1
    • Casein kinase I isoform alpha
    • CK1
    • CK1A
    • CKI alpha
    • CKI-alpha
    • CKIa
    • Clock regulator kinase
    • Csnk1a1
    • Down regulated in lung cancer
    • HLCDGP1
    • KC1A_HUMAN
    • PRO2975
    see all
  • 功能Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.
  • 序列相似性Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus speckle. Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis.
  • Information by UniProt

Recombinant human Casein Kinase 1 alpha protein 图像

  • ab102102 was determined to be >80% pure by densitometry. MWt 62 kDa.
  • The specific activity of ab102102 was determined to be 120 nmol/min/mg.

Recombinant human Casein Kinase 1 alpha protein (ab102102)参考文献

ab102102 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab102102.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"