Recombinant人CaMKII delta protein (ab84552)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • AccessionQ13557
    • 种属Human
    • 分子量82 kDa including tags
    • 氨基酸1 to 499
    • 标签GST tag N-Terminus

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技术指标

Our Abpromise guarantee covers the use of ab84552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The specific activity of ab84552 was determined to be 5860 nmol/min/mg.
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

    Other

  • 形式Liquid
  • 补充说明

    ab188554 (CaMKII peptide) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Calcium / calmodulin dependent protein kinase 2 delta
    • Calcium / calmodulin dependent protein kinase II delta
    • calcium/calmodulin-dependent protein kinase (CaM kinase) II delta
    • calcium/calmodulin-dependent protein kinase type II delta chain
    • Calcium/calmodulin-dependent protein kinase type II subunit delta
    • CAM kinase 2 delta
    • CAM kinase II delta
    • CaM kinase II delta subunit
    • CaM kinase II subunit delta
    • CaM-kinase II delta chain
    • CAMK 2d
    • CaMK-II delta subunit
    • CaMK-II subunit delta
    • CAMK2D
    • CAMKD
    • CAMKI
    • KCC2D_HUMAN
    • RATCAMKI
    see all
  • 功能CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release.
  • 组织特异性Expressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt

Recombinant human CaMKII delta protein 图像

  • Kinase Assay. Specific activity 5860 nmol/min/mg.
  • SDS-PAGE of ab84552. Approximate MWt 77 kDa.

Recombinant human CaMKII delta protein (ab84552)参考文献

ab84552 has not yet been referenced specifically in any publications.

Product Wall

The substrate used with (ab84552) Active human CaMKII delta full length protein is the synthetic peptide KKALRRQETVDAL-amide. The peptide is diluted in distilled water to 1mg/ml. Normally use around 10ul of diluted kinase for a single kinase assay.

The recombinant protein also includes an N-terminal tag which has a molecular weight of 26kDa. This is the reason we expect to see ab84552 to produce a band near 72kDa. Thanks!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"