Recombinant人CaMKII alpha protein (ab60899)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human
    • 序列MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEF MATITCTRFT EEYQLFEELG KGAFSVVRRC VKVLAGQEYA AKIINTKKLS ARDHQKLERE ARICRLLKHP NIVRLHDSIS EEGHHYLIFD LVTGGELFED IVAREYYSEA DASHCIQQIL EAVLHCHQMG VVHRDLKPEN LLLASKLKGA AVKLADFGLA IEVEGEQQAW FGFAGTPGYL SPEVLRKDPY GKPVDLWACG VILYILLVGY PPFWDEDQHR LYQQIKAGAY DFPSPEWDTV TPEAKDLINK MLTINPSKRI TAAEALKHPW ISHRSTVASC MHRQETVDCL KKFNARRKLK GAILTTMLAT RNFSGGKSGG NKKSDGVKES SESTNTTIED EDTKVRKQEI IKVTEQLIEA ISNGDFESYT KMCDPGMTAF EPEALGNLVE GLDFHRFYFE NLWSRNSKPV HTTILNPHIH LMGDESACIA YIRITQYLDA GGIPRTAQSE ETRVWHRRDG KWQIVHFHRS GAPSVLPH
    • 分子量74 kDa
    • 标签GST tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab60899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    256 nmol/min/mg

  • 应用

    Functional Studies

    SDS-PAGE

    Western blot

  • 形式Liquid
  • 补充说明

    ab188554 (CaMKII peptide) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Alpha CaMKII
    • Calcium calmodulin dependent protein kinase II
    • Calcium/calmodulin dependent protein kinase II alpha B subunit
    • Calcium/calmodulin dependent protein kinase type II alpha chain
    • Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
    • Calcium/calmodulin-dependent protein kinase II alpha
    • Calcium/calmodulin-dependent protein kinase II-alpha
    • Calcium/calmodulin-dependent protein kinase type II subunit alpha
    • Calcium/calmodulin-dependent protein kinase type IIA
    • CaM kinase II alpha chain
    • CaM kinase II alpha subunit
    • CaM kinase II subunit alpha
    • CaMK II alpha subunit
    • CaMK-II subunit alpha
    • Camk2a
    • CAMKA
    • CaMKII
    • CaMKIINalpha
    • EC 2.7.11.17
    • KCC2A_HUMAN
    • KIAA0968
    • MGC123320
    • MGC139375
    • MGC155201
    • mKIAA0968
    • PK2CDD
    • PKCCD
    • R74975
    • zgc:112538
    • zgc:123320
    see all
  • 功能CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • 细胞定位Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
  • Information by UniProt

Recombinant human CaMKII alpha protein 图像

  • Sample Kinase Activity Plot.
  • ab60899 on SDS-PAGE, MW ~74 kDa.

Recombinant human CaMKII alpha protein (ab60899)参考文献

This product has been referenced in:
  • Yuen EY  et al. Restoration of glutamatergic transmission by dopamine D4 receptors in stressed animals. J Biol Chem 288:26112-20 (2013). Read more (PubMed: 23884421) »

See 1 Publication for this product

Product Wall

For the activity assay the lab followed the following procedure:

1. 10 ul of diluted ab60899

2. 7.5 ul of 1 mg/ml substrate (Autocamtide 2 synthetic peptide substrate (KKALRRQETVDAL-amide) diluted in dH2O

3. 2.5 ul of Ca2+/Ca...

Read More

ab60899 has not been modified in any way to make it permanently active in the absence of calcium. However, it is possible that this protein, like other CAMK2a proteins, can autophosphorylate itself in the absence of Ca2+/Calmodulin, making it active, s...

Read More

Thank you for contacting us. Sandwich ELISA procedures can be difficult to optimize and tested match pair antibodies should be used. This ensures the antibodies are detecting different epitopes on the target protein so they do not interfere wit...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"