The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. ab116467 is purified by proprietary chromatographic techniques. Purity is greater than 90.0% as determined by RP-HPLC and SDS-PAGE analysis.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
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Shipped at 4°C. Please see notes section.
pH: 7.50 Constituent: 0.32% Tris HCl
calmodulin 1 (phosphorylase kinase, delta)
Calmodulin 2 (phosphorylase kinase, delta)
Calmodulin 3 (phosphorylase kinase, delta)
phosphorylase kinase delta
phosphorylase kinase, delta subunit
Function: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
has not yet been referenced specifically in any publications.