Recombinant人c Abl protein (ab69810)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • AccessionP00519
    • 种属Human
    • 分子量135 kDa
    • 氨基酸27 to 1130
    • 标签His tag N-Terminus

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技术指标

Our Abpromise guarantee covers the use of ab69810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    Specific activity: 871 nmol/min/mg

  • 应用

    Functional Studies

    SDS-PAGE

  • 形式Liquid
  • 补充说明

    ab204848 (c Abl peptide) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Abelson murine leukemia viral oncogene homolog 1
    • Abelson tyrosine protein kinase 1
    • ABL
    • Abl 1
    • ABL proto oncogene 1 non receptor tyrosine kinase
    • ABL1
    • ABL1_HUMAN
    • bcr/abl
    • bcr/c abl oncogene protein
    • c ABL
    • c abl oncogene 1 non receptor tyrosine kinase
    • c abl oncogene 1 receptor tyrosine kinase
    • c ABL1
    • JTK7
    • p150
    • Proto oncogene tyrosine protein kinase ABL1
    • Proto-oncogene c-Abl
    • Tyrosine-protein kinase ABL1
    • v abl
    • v abl Abelson murine leukemia viral oncogene homolog 1
    see all
  • 功能Protein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.
  • 组织特异性Widely expressed.
  • 疾病相关Note=A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻译后修饰Phosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation.
    Isoform IB is myristoylated on Gly-2.
  • 细胞定位Cytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear.
  • Information by UniProt

Recombinant human c Abl protein 图像

  • Kinase activity assay of ab69810. The specific activity of c-Abl was determined to be 871 nmol/min/mg.
  • SDS-PAGE of ab69810. Molecular weight 135kDa.

Recombinant human c Abl protein (ab69810)参考文献

ab69810 has not yet been referenced specifically in any publications.

Product Wall

This protein corresponds to amino acids 27-1130 of the human protein. It also includes 9 amino acids of a His tag fused to the N-terminus.

This protein should therefore been seen at ˜135 kDa on a blot. It is not a mutated version of the prote...

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Yes, HEPES pH 7.2 can be substituted for MOPS in the kinase assay buffer, at the same concentration, 25mM.

Please find attached the functional assay (kinase activity) for ab69810, which includes buffer recommendations.

Please let me know if you have any questions.

Thank you for your message.

I apologize for any confusion. I have checked with our products department who have confirmed that this was a duplicate in our catalog, ab84546 and ab69810 are both the same.

We have therefore removed a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"