重组人ABL1蛋白(ab69810)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Affinity purified
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人ABL1蛋白
参阅全部 ABL1 蛋白酶 -
生物活性
Specific activity: 871 nmol/min/mg
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纯度
> 70 % Affinity purified.
Purified by affinity chromatography -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
EALQRPVASD FEPQGLSEAA RWNSKENLLA GPSENDPNLF VALYDFVASG DNTLSITKGE KLRVLGYNHN GEWCEAQTKN GQGWVPSNYI TPVNSLEKHS WYHGPVSRNA AEYLLSSGIN GSFLVRESES SPGQRSISLR YEGRVYHYRI NTASDGKLYV SSESRFNTLA ELVHHHSTVA DGLITTLHYP APKRNKPTVY GVSPNYDKWE MERTDITMKH KLGEGHFGEV YEGVWKKYSL TVAVKTLKED TMEVEEFLKE AAVMKEIKHP NLVQLLGVCT REPPFYIITE FMTYGNLLDY LRECNRQEVN AVVLLYMATQ ISSAMEYLEK KNFIHRDLAA RNCLVGENHL VKVADFGLSR LMTGDTYTAH AGAKFPIKWT APESLAYNKF SIKSDVWAFG VLLWEIATYG MSPYPGIDLS QVYELLEKDY RMERPEGCPE KVYELMRACW QWNPSDRPSF AEIHQAFETM FQESSISDEV EKELGKQGVR GAVSTLLQAP ELPTKTRTSR RAAEHRDTTD VPEMPHSKGQ GESDPLDHEP AVSPLLPRKE RGPPEGGLNE DERLLPKDKK TNLFSALIKK KKKTAPTPPK RSSSFREMDG QPERRGAGEE EGRDISNGAL AFTPLDTADP AKSPKPSNGA GVPNGALRES GGSGFRSPHL WKKSSTLTSS RLATGEEEGG GSSSKRFLRS CSASCVPHGA KDTEWRSVTL PRDLQSTGRQ FDSSTFGGHK SEKPALPRKR AGENRSDQVT RGTVTPPPRL VKKNEEAADE VFKDIMESSP GSSPPNLTPK PLRRQVTVAP ASGLPHKEEA GKGSALGTPA AAEPVTPTSK AGSGAPGGTS KGPAEESRVR RHKHSSESPG RDKGKLSRLK PAPPPPPAAS AGKAGGKPSQ SPSQEAAGEA VLGAKTKATS LVDAVNSDAA KPSQPGEGLK KPVLPATPKP QSAKPSGTPI SPAPVPSTLP SASSALAGDQ PSSTAFIPLI STRVSLRKTR QPPERIASGA ITKGVVLDST EALCLAISRN SEQMASHSAV LEAGKNLYTF CVSYVDSIQQ MRNKFAFREA INKLENNLRE LQICPATAGS GPAATQDFSK LLSSVKEISD IVQR -
预测分子量
135 kDa -
氨基酸
27 to 1130 -
标签
His tag N-Terminus
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab69810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
ab204848 (ABL1 peptide) can be utilized as a substrate for assessing Kinase activity
Previously labelled as c Abl.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Abelson murine leukemia viral oncogene homolog 1
- Abelson tyrosine protein kinase 1
- ABL
see all -
功能
Protein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. -
组织特异性
Widely expressed. -
疾病相关
Note=A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
翻译后修饰
Phosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation.
Isoform IB is myristoylated on Gly-2. -
细胞定位
Cytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear. - Information by UniProt
图片
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The specific activity of ABL1 (ab69810) was determined to be 765.8 nmol/min/mg as per activity assay protocol
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SDS PAGE analysis of ab69810
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SDS PAGE analysis of ab69810
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Kinase activity assay of ab69810. The specific activity of c-Abl was determined to be 871 nmol/min/mg.
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SDS-PAGE of ab69810. Molecular weight 135kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab69810 被引用在 1 文献中.
- Campbell H et al. SHP-2 is activated in response to force on E-cadherin and dephosphorylates vinculin Y822. J Cell Sci 131:N/A (2018). PubMed: 30478196