Recombinant人BTK protein (ab42616)



  • 性质
  • 来源
    Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属
    • 分子量
      106 kDa
    • 标签
      proprietary tag N-Terminus


Our Abpromise guarantee covers the use of ab42616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    Specific Activity : >450 U/mg. One unit defined as the amount of enzyme that will transfer 1nmol phosphate to Tyr substrate per minute at pH 7.4 and 30deg.C. Assay buffer : 50mM HEPES pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3um Na-orthovanadate, 0.1M ATP, 30ug/ml Poly (Glu:Tyr) 4:1 substrate and 0.75ug/ml recombinant BTK.
  • 应用

    Inhibition Assay

  • 形式
  • 补充说明
    This protein was expressed in Baculovirus infected Sf9 cells.
  • Concentration information loading...


  • 稳定性和存储

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 30% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, 10mM reduced Glutathione, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.


  • 别名
    • Agammaglobulinaemia tyrosine kinase
    • AGMX 1
    • AGMX1
    • AT
    • ATK
    • B cell progenitor kinase
    • B-cell progenitor kinase
    • BPK
    • Bruton agammaglobulinemia tyrosine kinase
    • Bruton tyrosine kinase
    • Bruton’s Tyrosine Kinase
    • Btk
    • dominant-negative kinase-deficient Brutons tyrosine kinase
    • IMD 1
    • IMD1
    • MGC126261
    • MGC126262
    • OTTHUMP00000063593
    • PSCTK 1
    • PSCTK1
    • truncated Bruton agammaglobulinemia tyrosine kinase
    • Tyrosine protein kinase BTK
    • Tyrosine-protein kinase BTK
    • tyrosine-protein kinase BTK isoform (lacking exon 14
    • XLA
    see all
  • 功能
    Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes.
  • 疾病相关
    Defects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
    Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD).
  • 序列相似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
    Contains 1 Btk-type zinc finger.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻译后修饰
    Autophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein.
  • 细胞定位
    Cytoplasm. Membrane. Nucleus.
  • Information by UniProt

Recombinant human BTK protein (ab42616)参考文献

ab42616 has not yet been referenced specifically in any publications.

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